Stripping Hybond membranes

[Rachelle Leger]

I seem to loose material from my blot when I strip Hybond N Plus membranes
Is is difficult to know for sure that this is really happening but when I
reuse it the next time I need to expose much longer.
Is this your experience?
I strip by pouring boiling .1% SDS and .1% SSC on the membrane and skaking 
for 10 minutes.

Thanks for any response

Rachelle Leger
McGill Cancer Centre
McGill University

Leger at medcor.mcgill.ca
ax401 at freenet.carleton.ca