Stripping Hybond membranes

Rachelle Leger ax041 at FreeNet.Carleton.CA
Mon Jul 3 06:07:11 EST 1995


I seem to loose material from my blot when I strip Hybond N Plus membranes
Is is difficult to know for sure that this is really happening but when I
reuse it the next time I need to expose much longer.
Is this your experience?
I strip by pouring boiling .1% SDS and .1% SSC on the membrane and skaking 
for 10 minutes.

Thanks for any response

Rachelle Leger
McGill Cancer Centre
McGill University

Leger at medcor.mcgill.ca
ax401 at freenet.carleton.ca



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