Stripping Hybond membranes

Robert D. C. Saunders rdcsaunders at anatphys.dundee.ac.uk
Mon Jul 3 16:58:11 EST 1995


In article <DB507z.B13 at freenet.carleton.ca> ax041 at FreeNet.Carleton.CA (Rachelle Leger) writes:
>From: ax041 at FreeNet.Carleton.CA (Rachelle Leger)
>Subject: Stripping Hybond membranes
>Date: Mon, 3 Jul 1995 11:07:11 GMT

>I seem to loose material from my blot when I strip Hybond N Plus membranes
>Is is difficult to know for sure that this is really happening but when I
>reuse it the next time I need to expose much longer.
>Is this your experience?
>I strip by pouring boiling .1% SDS and .1% SSC on the membrane and skaking 
>for 10 minutes.

This has been my experience too.  Both with N and N+.  Furthermore I have 
suffered this with other nylon membranes.  I use nitrocellulose wherever 
possible.

Robert



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