Sequencing AT rich regions

Shin-ichiro HIRAGA shiraga at gen-info.osaka-u.ac.jp
Wed Jul 5 00:08:56 EST 1995


I think that the optimum temp of Tth pol is 75 degree C. I'm not sure 
but the local AT rich region may be denatured at that temp. I think you 
should try T7 pol or Sequanase system for the region.

Shin-ichiro Hiraga



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