Is lambda EMBL 3A suppressed by supE?

John Dixon jpcd0 at
Wed Jul 5 06:55:32 EST 1995

Can anyone tell me if the amber mutations in lambda EMBL 3A (Aam Bam) are
suppressed by supE as well as supF?

Are the mutations overcome by prolonged incubation? I was using lambda ZAP
(Sam100) and if the lambda are incubated >8hrs all form plaques on sup0
hosts. Can this be a problem for AamBam lambda?

Thanks in advance

Johnny D

John Dixon                     Lab 44 (223) 334131
Wellcome/CRC Institute         Fax 44 (223) 334134
Dept Genetics
Cambridge University    
United Kingdom       e-m: jpcd0 at

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