Is lambda EMBL 3A suppressed by supE?

[John Dixon]

Can anyone tell me if the amber mutations in lambda EMBL 3A (Aam Bam) are
suppressed by supE as well as supF?

Are the mutations overcome by prolonged incubation? I was using lambda ZAP
(Sam100) and if the lambda are incubated >8hrs all form plaques on sup0
hosts. Can this be a problem for AamBam lambda?

Thanks in advance

Johnny D

-- 
John Dixon                     Lab 44 (223) 334131
Wellcome/CRC Institute         Fax 44 (223) 334134
Dept Genetics
Cambridge University    
United Kingdom       e-m: jpcd0 at mole.bio.cam.ac.uk