How to remove albumin?

Tomas Bratt tbratt at sbsnov1.auckland.ac.nz
Tue Jul 4 19:49:40 EST 1995


In article <Morty.144.2FF87327 at bchm.unp.ac.za>, Morty at bchm.unp.ac.za
(Rory.Morty) wrote:

> Hi there!
> 
> Any ideas about how to COMPLETELY remove trace amounts of albumin from an 
> otherwise pure preparation of protein. MEC is not feasable because the 
> molecular weight of the protein of interest is too close to 68 kDa. The 
> trace amounts of albumin have not been removed by a number of IEC steps, or 
> chromatography on Cibacron Blue or phenyl sepharose. Any ideas?
> 
> Rory Morty
> e-mail: morty at gate2.cc.unp.ac.za

Try protein G affinity chromatography, but be sure that the albumin
binding part of protein G has not been deleted in the protein G you use.
Your protein will pass through and the albumin could be eluted and protein
G used again

Tomas

-- 
David Franklin, Auckland, New Zealand



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