How to remove albumin?
tbratt at sbsnov1.auckland.ac.nz
Tue Jul 4 19:49:40 EST 1995
In article <Morty.144.2FF87327 at bchm.unp.ac.za>, Morty at bchm.unp.ac.za
> Hi there!
> Any ideas about how to COMPLETELY remove trace amounts of albumin from an
> otherwise pure preparation of protein. MEC is not feasable because the
> molecular weight of the protein of interest is too close to 68 kDa. The
> trace amounts of albumin have not been removed by a number of IEC steps, or
> chromatography on Cibacron Blue or phenyl sepharose. Any ideas?
> Rory Morty
> e-mail: morty at gate2.cc.unp.ac.za
Try protein G affinity chromatography, but be sure that the albumin
binding part of protein G has not been deleted in the protein G you use.
Your protein will pass through and the albumin could be eluted and protein
G used again
David Franklin, Auckland, New Zealand
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