SOB Medium: Why add Mg late?

Michael Deyholos mdeyholo at
Tue Jul 4 15:01:36 EST 1995

Just curious why Sanbrook/Maniatis reccommend autoclaving SOB, then 
adding MgCl2, then re-autoclaving.  Why not add the MgCl2 to the original 
soltuion prior to autoclaving?

Mike Deyholos
McGill Dept. of Biology

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