pCDNAI and pCDM8 problems in stable transfection ?

[marcvdc at lmb1.rug.ac.be]

Dear Netters,


It seems that there are problems with the stable transfections of genes 
cloned in pCDM8.  Although the transient transfections of the gene gave 
high expression levels, no protein was detected in the stable transfectans.  
The same was done with an biological inactive form of the protein and this 
gave the same results. So, it is not because of the toxicity of the gene 
expressed.

Now I wonder wether someone had the same problems with pCDM8.  Are the same 
problems seen in the pCDNAI (in vitrogen)?  Maybe, I will use this vector in 
the future but then I want to be sure not to encounter the same problems.

Please respond also to me personal.

Thanks,

Marc