Call for LacZ fragment for terminal bash
brad at corona.med.utah.edu
Wed Jul 5 09:46:06 EST 1995
In article <Pine.SGI.3.91.950703161353.19120A-100000 at bgumail.bgu.ac.il>,
raveh at BGUMAIL.BGU.AC.IL (Dina Raveh BGU) wrote:
> We have a protein whose DNA sequence ends at +2. Does anyone have a lacZ
> gene which we could blunt onto this and get readthru?
> Thanks, Dina
You might look for someone who has one of the vectors from Robert Simons,
pRS415, pRS551 etc. He has both protein and operon fusions to lacZ, with a
multi-cloning site with EcoRI-SmaI-BamHI (both orientations), it also has a
transcriptional terminator in front of the cloning sites to prevent read-
through. This may work for you. The reference is:
Simons, R., Houman, F. & Kleckner, N.. 1987, Improved single and multicopy
lac-based cloning vectors for protein and operon fusions. Gene, 53, 85-96.
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