klenow

[vdrover at kean.ucs.mun.ca]

just a quick question...i want to end-fill restriction fragments (ie:lamda 
DNA cut with HindIII) to incorporate 32P-dNTPs. I've got Klenow from 
pharmacia but the buffers for the reaction vary from place to place. Does 
anyone have a reliable buffer (component concentrations as well) to us with 
Klenow? please mail responses to vdrover at kean.ucs.mun.ca

vic drover
memorial university of newfoundland
st. john's, newfoundland
canada