HELP with glcyerol-tolerant sequencing gels

SquireNige squirenige at
Wed Jul 5 20:27:55 EST 1995


I think this deal with the glyceroo tolerant buffer is somewaht of a
misnomer. I routinely have run 5% gels using TBE having diluted sequenase
in the glycerol buffer and i dont seem to have any problems. The artifact
caused by the glycerol  runs up in the 400 bp region so for shorter runs
than this I wouldnt worry about it. It doesnt seem as pronounced using the
5% gesl either so that may be a way out for you?

Good luck


nwalker at
Johns Hopkins University

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