Plasmid sequencing: Methods to improve resolution

S941407 at mailserv.cuhk.hk S941407 at mailserv.cuhk.hk
Thu Jul 6 16:23:48 EST 1995


I would like to know how I can get better-resolution sequencing (bands 
towards the end of the gel are hardly distinguished). Moreover, are there any 
methods to increase the number of sequence obtained each time(other than 
buffer-gradient page)?

Information about my sequencing procedures:

I am now using T-7 sequencing kit with S35 dATP label. I have cloned my PCR 
product into PCR II vector before performing sequencing.  

Thanks a lot!

Frankie, T. C. Lee




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