Plasmid sequencing: Methods to improve resolution
S941407 at mailserv.cuhk.hk
S941407 at mailserv.cuhk.hk
Thu Jul 6 16:23:48 EST 1995
I would like to know how I can get better-resolution sequencing (bands
towards the end of the gel are hardly distinguished). Moreover, are there any
methods to increase the number of sequence obtained each time(other than
buffer-gradient page)?
Information about my sequencing procedures:
I am now using T-7 sequencing kit with S35 dATP label. I have cloned my PCR
product into PCR II vector before performing sequencing.
Thanks a lot!
Frankie, T. C. Lee
More information about the Methods
mailing list