Pre-flashing films for ECL??

Darren Fast fast at
Thu Jul 6 10:02:07 EST 1995

Hi there, I would like to get the collective wisdom on the requirements
for pre-flashing films for quantitation of ECL signals.  Does pre-flashing
improve linearity of signal?  I would like to quantitate some western
blots.  Any other info or discussion of western quantitation would be

Also, if pre-flashing of films is important, how does one go about
creating a flash with the correct exposure?  Are there commercial
pre-flash units available?


Darren Fast
fast at
Dept of Biochemistry
University of Wisconsin-Madison

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