Brennan S Sawyer
bsawyer at SELWAY.UMT.EDU
Fri Jul 7 13:25:29 EST 1995
Our lab has been working with 4-thioUTP for some time, and we have been
continually debating about the reactivity of this molecule. Will
4-thioUTP cross-link in visible light? If it is left in a clear eppie
tube on the bench top will it form cross-links? If so, how much light
exposure is too much? Will brown eppie tubes cut down or prevent such
cross-linking? I should mention we are using T7 RNA pol to transcribe
small RNAs that contain 4-thioUs at various positions.
We regularly work with tRNA-Phe from E.coli which has a 4thioU at
position 8, and we never worry about this tRNA forming cross-links.
Also, could some one give a brief explanation of the mechanism of
cross-linking, we are not cross-linkers by trade.
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