how to work out curvature of DNA--part two???
gqva12 at udcf.gla.ac.uk
Thu Jul 6 02:32:28 EST 1995
hello again !!!
sorry for the loose terminology in my previous post and thanks to those
who emailed me so quickly.
In a paper (Immunogenetics, vol.39, 150-154, 1994), D'Alfonso and Richiardi
describe the effect of a single base substitution in a putative Y-box, in the
human TNF promoter. One of the things they note is that the change of G/A
results in a *subtle* change in (quote) "..variation at position -238 slightly
affects DNA curvature is also shown by the images obtained by a computer program
that predicts DNA superhelical conformation from nucleotide sequence.."
The reference they give for this is De Santis et al, Biophys Chem., vol32
I can't get hold of this programme and anyway, thought there might be a more
Secondly, I am aware of papers (again from the late 80's) that describe
"promoter" function of dinucleotide repeats (eg Hamada et al, Mol Cell Biol,
vol?? december 1984, pp2610 - 2621) which seems to be ascribed to the possible
change to z-DNA that such repeats might try to achieve.
I should say that I am a cellular immunologist and so am not up to date with
research in either of these areas, which is sort of why I asked the board..
Anyway, like I said, I'm interested in the possible efects of changing the
size of a dinucleotide (CA) repeat and would welcome any thoughts.
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