Southern Hybridization Buffers?

veronica narvaez v-narvae at nimr.mrc.ac.uk
Fri Jul 7 09:54:11 EST 1995


Hello Everybody!
I'm going to start doing southerns, and I found there are a lot of different
hybridization buffers, ie, some use a different prehybridization solution
than the hybridization one. Some use Denhardt, some not, etc.
Any preferred hybridiation solution? A nice, reliable, easy one?
Any of you heard of "Church"  hybridization buffer? Is it good? Can 
you give me the recipe?
Thank you very much!
Veronica



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