Cat Ig mRNA detection

Sebastian Bunka bunka at
Fri Jul 7 08:27:44 EST 1995


we are looking for a method for the detection of cat immunoglobulin mRNA
in mouse-cat heterohybridomas. We have a few clones that produced for a
very short time cat immunoglobulins. We now have to check if mRNA for
cat Ig's is produced at all. We are thinking of some kind of RT-PCR.
Our myeloma cell line doesn't produce Ig mRNA!

Any suggestions for the source of primers that are directed against
highly conserved sequences of the light or heavy chain genes that will
work for cat chromosomes, too? 

Thanks in advance,

Cheers, Sebastian


Sebastian Bunka			ph. (+43-1) 711 55 301
Institute of			FAX (+43-1) 713 68 95
Medical Chemistry		email: Sebastian.Bunka at
University of Veterinary Medicine - Vienna - Austria

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