Help: COncanavalin A chromatography
Morty at bchm.unp.ac.za
Wed Jul 5 15:47:46 EST 1995
I have a question about affinity chromatography with Concanavalin A (Con A).
Once the sample intended for fractionation has been applied to and eluted
from the Con A column, say with methyl mannoside or methyl glucoside, how
should the column be treated before the next sample is applied? Do the now
tightly bound mannosides and glucosides have to be removed from the column
before the next sample is applied, and if so, how?
Many thank's in advance,
e-mail: morty at gate2.cc.unp.ac.za
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