Glycerol loading buffer in acrylamide gels?

Zorg Zorg at berkeley.edu
Sat Jul 8 22:54:25 EST 1995


Hi fellow bionetters,
     I am planning to run some 0.4 mm nondenaturing polyacrylamide gels. 
I have heard (and I can't remember from where) that too high a
concentration of glycerol in the sample can interfere with resolution when
run in 1X TBE.  I was wondering why this was the case and, more
importantly, whether Ficoll or sucrose would serve as an adequate
substitute.  
     My apologies if this matter has been addressed in the past ('tho I
did check :)

Thanking you in advance,
Gene Huh
gshuh at garnet.berkeley.edu



More information about the Methods mailing list