Direct PCR from E. coli colonies

[Graham Dellaire]

>   mytelka at mendel.Berkeley.EDU ( Daniel Mytelka ) writes:
>  I actually do it from liquid cultures, but it can be adapted for
>  colonies. 
>  
>  Spin down 0.5 ml of an overnight.
>  
>  Resuspend in 1 ml water, spin down again.
>  
>  Resuspend in 1 ml water.
>  
>  Transfer 50 ul to a small tube, incubate 5' at 95C.
>  
>  Add 50 ul 2x PCR reaction mix, overlay with oil, PCR.
>  
>  
>  
>  Daniel Mytelka
>  mytelka at mendel.berkeley.edu 
>  
>>>>