Direct PCR from E. coli colonies

Daniel Mytelka mytelka at mendel.Berkeley.EDU
Sun Jul 9 05:20:37 EST 1995


I actually do it from liquid cultures, but it can be adapted for
colonies. 

Spin down 0.5 ml of an overnight.

Resuspend in 1 ml water, spin down again.

Resuspend in 1 ml water.

Transfer 50 ul to a small tube, incubate 5' at 95C.

Add 50 ul 2x PCR reaction mix, overlay with oil, PCR.



Daniel Mytelka
mytelka at mendel.berkeley.edu 



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