Southern-PCR

[Graham Dellaire]

You could try just vortexing  the membrane with TE and then amplifying the
supernatent after pelleting the membrane.  You can try it.  I have amplified DNA
from Nitrocellulose paper from an electroelution but that is it.  Maybe the crosslinking of the
DNA to the membrane will cause you problems..... Do you have a positive control for your primers
or are you on a fishing expedition?



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