Home-Made TA cloning

Tjian lab insomnia at mendel.berkeley.edu
Sun Jul 9 19:28:25 EST 1995


s060965 at AIX1.UOTTAWA.CA wrote:
>I've been trying to think of a way to make my own TA cloning kit.  
>
{Stuff deleted}

>Any ideas out there.
>
>Cris Martin

Actually, it's a lot easier than that.  Just linearize bluescript with 
EcoRV and incubate with Taq and dTTP at 72 deg for 2 hrs.  The terminal 
transferase activity is non-specific and will use dTTP if nothing else 
is available.  See Marchuk et al, NAR 19(5):1154 for details

Shane Albright
MCB Dept
UC Berkeley





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