Home-Made TA cloning
insomnia at mendel.berkeley.edu
Sun Jul 9 19:28:25 EST 1995
s060965 at AIX1.UOTTAWA.CA wrote:
>I've been trying to think of a way to make my own TA cloning kit.
>Any ideas out there.
Actually, it's a lot easier than that. Just linearize bluescript with
EcoRV and incubate with Taq and dTTP at 72 deg for 2 hrs. The terminal
transferase activity is non-specific and will use dTTP if nothing else
is available. See Marchuk et al, NAR 19(5):1154 for details
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