Does size matter? dsDNA IS probes

Robert Pierce rpierce at
Sun Jul 9 21:17:21 EST 1995

I'm trying to make double stranded DNA probe utilizing BM's digoxigenin 
incorporated dUTP and RT-PCR off a plasmid containing cDNA.  I 
can choose primers adequate for the PCR in virtually any region, yielding 
whatever probe size is optimal.  But what is optimal?  Any input 
regarding this question and helpful hints about the Dig-dUTP system will 
be appreciated.  Thanks in advance.


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