losing overhangs over time?

John Dixon jpcd0 at mole.bio.cam.ac.uk
Sun Jul 9 18:47:10 EST 1995


Invitrogen seem to insist that you need to buy a new TA cloning kit after
a month or so because the terminal T's fall off or degrade or something
and leave a blunt ended and inefficient vector.

Is this true? And if so does this phenomenon affect other gel purified
fragments? Will 4-base overhangs slowly degrade into blunts?

Anyone who has cloned using an old supply of cut vector can probably tell
me (I always make mine fresh).

TIA, Johnny D

John Dixon                     Lab 44 (223) 334131
Wellcome/CRC Institute         Fax 44 (223) 334134
Dept Genetics
Cambridge University    
United Kingdom       e-m: jpcd0 at mole.bio.cam.ac.uk

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