Accurate and Long PCR

Sasha Kraev kraev at bc.biol.ethz.ch
Mon Jul 10 07:09:01 EST 1995


We have recently solved exactly the same problem ( cloning a ca.3 kb
ORF after mutagenesis ), by using Boehringer Expand kit. The complete
ORF could not be amplified with regular Taq from the same manufacturer,
but the Taq/Pwo mixture worked immediately! Hope this helps.





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