Home-Made TA cloning
Mon Jul 10 07:46:04 EST 1995
>> I've been trying to think of a way to make my own TA cloning kit.
>> There doesn't seem to be any available restriction enzymes that will
>> produce the proper ends for TA cloning (although I'm sure Invitrogen
>> have one in their bag of tricks).
I am sure that Invitrogen is probably making their TA vector by first
linearizing the polylinker with EcoR1 and then ligating an EcoR1 linker
containing a T overhang.
eg: EcoR1 side
5' AATTCGGCTT 3'
Such a linker would be easy to make and fairly cheap since it is so
short. Unfortunately, I can't say that this method works as well in the
lab as it does on paper because I am lazy and just buy the kit.
Christopher D. Skory
Agricultural Research Service
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