scontz at passport.ca
Mon Jul 10 21:24:08 EST 1995
I am currently expressing and purifying a recombinant protein, calculated
17 kDa, with the pET-30 expression system from Novagen. The system uses a
hexahistidine stretch and Ni-affinity chromatography for purification. It
seems that I have a large yeild, and now concentration is a problem. I am
currently using Ultrafree-20 low binding cellulose filters from Millipore,
but I seem to lose a large quantity of my target protein. Does anyone have
any ideas or tricks that I may try to concentrate my protein prep to
roughly 1 ml? Has anyone tried using dialysis and polyethylene glycol or
Sephadex? Any help would be greatly appreciated
Copyright 1995 Elmer Fudd.
All wights wesewved.
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