cleaning up IEF tubes?

John A. Newitt newitt at ncifcrf.gov
Mon Jul 10 13:04:05 EST 1995


In article <gurer-070795112242 at gellab.life.uiuc.edu>,
gurer at pop.life.uiuc.edu (Chaan Gurer) wrote:

> Does anyone know a reliable protocol for cleaning up IEF tubes after the
> gels
> are pushed out. We ususally rinse them with water and wash them with
> chromic
> acid. However, lately we have had some uneven polymerization incidents in
> our
> tubes which might be an indication of dirty tubes. Is there another way to
> clean the tubes? Thanks.
 
We used to soak them in 50% Nitric acid in water.  I don't know if this
method is any better than the one you are currently using, but it might be
worth a try.

Regards,
        John A. Newitt, Ph.D.           |     <newitt at nih.gov>
        National Institutes of Health   |     <newitt at ncifcrf.gov>
        Bethesda, Maryland  USA         |     <73043.1773 at compuserve.com>




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