how leaky is it for lacIq?

Ing nang ingnang at life.cc.sunysb.edu
Mon Jul 10 13:38:06 EST 1995


Hi,

I wonder if any one can give me some clues on this, so I won't loose 
any sleep tonight :).    If you have two copies of lac promotors on the
plasmid, say, pBlueScript, and the host cell has lacIq mutation on it, how
tightly controlled the expression would be?  Could the over-produced lac
repressors be diluted by the presence of two promotors, so that the 
expression becomes leaky?

--
Ing-Nang Wang

Dept. Ecology and Evolution
SUNY-Stony Brook

ingnang at life.bio.sunysb.edu



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