Ligation in 5 min!

Guy Oshiro oshiro_G at defiance.hsc.colorado.edu
Mon Jul 10 13:51:59 EST 1995


Oops, looks like I have to rescind a little of my last post.
Seems that someone did do some quantitative transformations to test
ligation efficency.

>ligated at room temp,
>trafo 2 ul of ligation after
>10 min ---> 222 colonies (2/5 of transformation plated)
>35 min ---> 377 col.     (2/5 of transformation plated)
>73 min ---> 624 col.     (2/5 of transformation plated)
>22 h   ---> 680 col.     (2/5 of transformation plated)
>testliga without insert -> 22 col. (after 22 h)



Guy Oshiro
EMAIL: Oshiro_G at defiance.hsc.colorado.edu

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