Home-Made TA cloning

nsaunders at molbiol.ox.ac.uk nsaunders at molbiol.ox.ac.uk
Mon Jul 10 13:01:32 EST 1995


No, it must be dTTP. If it were ddTTP, you'ld have no free 3' OH group to form 
a phosphodiester bond to the A residue of the PCR insert and the ligation 
wouldn't work.

Neil

nsaunders at molbiol.ox.ac.uk
 



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