cleaning up IEF tubes?

Hiranya Roychowdhury hroychow at NMSU.EDU
Mon Jul 10 12:08:07 EST 1995


On Fri, 7 Jul 1995, Chaan Gurer wrote:

> Does anyone know a reliable protocol for cleaning up IEF tubes after the
> gels
> are pushed out. We ususally rinse them with water and wash them with
> chromic
> acid. However, lately we have had some uneven polymerization incidents in
> our
> tubes which might be an indication of dirty tubes. Is there another way to
> clean the tubes? Thanks.
> Chaan Gurer
> University of Illinois
> Howard Hughes Program
> 
> 

I clean them (following a thorough rinse in DI water) by soaking them O/N 
in conc. HNO3, in the hood using a 50 or 100mL cylinder. After this I 
rinse them in DI water with several changes, soak them for an hour in 95% 
EtOH, wash off the EtOH with several changes of ddH2O and dry in an oven.

Chromic acid should be OK, but has to be clean and relatively fresh. In
general Cr-acid baths tend to accumulate a lot of debris from repeated use
and those may stick to the inner surface of the narrow tubes. 
			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
			<<<<<<<<<<<<<<<<<<<<<<<<<<<




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