Wanted: Anti-LacI or HSV-VP16
bernard at elsie.nci.nih.gov
Tue Jul 11 15:11:01 EST 1995
In article <3ts4ac$17v at news.ycc.yale.edu>, Edward C. Goodwin, Ph.D. <ecg at po.cwru.edu> writes:
> I'm trying to use an inducable system based on a Lac Repressor-Herpes
> VP16 fusion protein. However, I really need to document expression of
> this protein since functional assays are a pain and need to be reserved
> for a few stable cell lines. A western would seem to be ideal but I am
> having an unexpectedly difficult time in tracking down antibodies to Lac
> I or VP16 (I've checked the usual sources without success). If you know
> of any such antibodies, or better yet have an excess that you'ld be
> willing to share, please drop me a line.
Stratagene sell an anti-LacI polyclonal for analysis of their Lac-Switch
expression plasmid. I have no idea how good it is as mine is still in
the freezer. Another possibility is Kathleen Shive Matthews at Rice
University who *had* monoclonal antibodies :-( and has polyclonal
antibodies against LacI.
Check out JBC 260,1185-1190 (1985) for her (very nice) work.
All the best,
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)
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