DNA sticking to glass

Robert Horton horton at biosci.cbs.umn.edu
Tue Jul 11 12:37:33 EST 1995


Chris Barry (chbarry at ucdavis.edu) wrote:
: Besides using siliconized glass, Is any one aware of a method 
: that will either prevent DNA from sticking to glass or a reagent that
: will release DNA bound to glass?

How 'bout a blocking agent, like tRNA, or BSA, or gelatin? These should 
be compatible with PCR, if you're trying to do that in glass. Otherwise, 
the traditional hybridization-type blocking agents (Denhardt's, nonfat 
dry milk, Bailey's Irish Creme Liquor) would be worth  a shot.

-Bob




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