pT7-7 cloning

Robert Horton horton at
Tue Jul 11 12:33:34 EST 1995

Chunlin (kamin_johnson at wrote:
: ... I found purified
: DNA solution did not frzee at -20C when glass beads was used to isolate
: DNA fragments from agarose gels. Could you help me find what could be
: worry in my experiments ?

I don't know what's in the Qiagen wash solution, but the one I use is an 
alcohol solution. If you carried too much of this over into your eluted 
DNA, it could keep it from freezing, and prevent subsequent enzymatic 
manipulation (like ligation).


More information about the Methods mailing list