pT7-7 cloning

Robert Horton horton at biosci.cbs.umn.edu
Tue Jul 11 12:33:34 EST 1995


Chunlin (kamin_johnson at cellbio.edu) wrote:
: ... I found purified
: DNA solution did not frzee at -20C when glass beads was used to isolate
: DNA fragments from agarose gels. Could you help me find what could be
: worry in my experiments ?

I don't know what's in the Qiagen wash solution, but the one I use is an 
alcohol solution. If you carried too much of this over into your eluted 
DNA, it could keep it from freezing, and prevent subsequent enzymatic 
manipulation (like ligation).

-Bob




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