Home-Made TA cloning

[Brian Desany]

There are restriction enzymes that leave single base overhangs in the 
middle of their site, XcmI (ccannnnn/nnnntgg) for example.  Two of these 
sites with the appropriate bases chosen for the "n"'s should make it 
really easy to TA clone - you don't have to ligate anything to the 
cleaved vector before you do your actual TA cloning reaction.  Although 
I haven't got around to trying it yet.