Protocol needed stain Cos cells for DNA,RNA
Mary P. Remington
mremingt at umabnet.ab.umd.edu
Tue Jul 11 06:17:36 EST 1995
We have transfected by electroporation, Cos-1 cells with an expression
vector containing a retroviral gene. Is there a way to probe cells on a
slide for DNA and RNA. We want to determine if the cells have been
transfected and if there is RNA being made from the construct. I know we
could isolate RNA and DNA and do Southerns and NOrtherns but, we are
looking for something quicker and in vitro. Thanks for any help.
Regards, Mary
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