SV40, large T antigen and transformed cells???

Alistair Forrest forrest at biosci.uq.oz.au
Tue Jul 11 01:32:22 EST 1995


G'day All,
	I'm trying to produce transformed cell lines, using pcDNA3 from
invitrogen. It has neo under SV40 and CMV for expression of the foreign gene.
What I'm trying to find out is, as the plasmid doesn't contain the SV40 T 
antigen, do I need to transform the plasmid into cells such as COS1 or 7 to
get stable expression of my insert or are these cells only used for episomal
replication?

Yours sincerely,
	Alistair Forrest

PhD Student



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