Sma I religation problem

[Mike Poidinger]

Hello everyone,

I have been having major troubles with religating Sma I cut pGEM.  The vector
has been cut, and the linear plasmid gel purified using Wizard clean up system,
but try as I might it wont religate to itself.  The same routine performed on
the same plasmid with Eco RI works just fine.

Any help anyone has would be greatly appreciated.

advTHANKSance,
Mike

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Dr Mike Poidinger      Now don't be lazy, 
Microbiology, UQ       with the pleasure of sin  (Nitzer Ebb)
Australia              
mikep at biosci.uq.oz.au  
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