Sma I religation problem

Mike Poidinger mikep at
Tue Jul 11 18:27:02 EST 1995

Hello everyone,

I have been having major troubles with religating Sma I cut pGEM.  The vector
has been cut, and the linear plasmid gel purified using Wizard clean up system,
but try as I might it wont religate to itself.  The same routine performed on
the same plasmid with Eco RI works just fine.

Any help anyone has would be greatly appreciated.


Dr Mike Poidinger      Now don't be lazy, 
Microbiology, UQ       with the pleasure of sin  (Nitzer Ebb)
mikep at  

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