DNA recovery from gels

bicfo at flinders.edu.au bicfo at flinders.edu.au
Tue Jul 11 14:46:26 EST 1995


Gidday, fellow netters!
I'm having truckloads of trouble trying to consistently recover DNA 
(0.7-1.8kb) from low-melting point agarose gels. I've tried agarase digestion, 
phenol extraction, Promega  WIZARD PCR columns  and 'squeeze-freeze' 
techniques - all with limited success (none at all with the agarase!). Does 
anybody have a foolproof method, or can anyone direct me to somewhere on the 
net where different techniques might be discussed?
Thanks, Colleen.



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