I cut sigmacote 1:10 with chloroform, apply evenly to one plate with a lint-free tissue, let air dry then assemble the gel plates for casting. To remove the "film" I use 5% KOH (w/v) in 100% methanol then was the plate as normal. Cheers Karl -- Karl Fischer kfischer at gpu.srv.ualberta.ca tyr-2 at bones.biochem.ualberta.ca