hekp with sigmacoat and sequencing gels

Karl Fischer kfischer at gpu.srv.ualberta.ca
Tue Jul 11 11:21:32 EST 1995


I cut sigmacote 1:10 with chloroform, apply evenly to one plate with a
lint-free tissue, let air dry then assemble the gel plates for casting. To
remove the "film" I use 5% KOH (w/v) in 100% methanol then was the plate
as normal.

Cheers 

Karl

-- 
Karl Fischer
kfischer at gpu.srv.ualberta.ca
tyr-2 at bones.biochem.ualberta.ca



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