Sma I religation problem

Roger Anderson rogera at
Wed Jul 12 00:27:46 EST 1995

Pierce recommends adding PEG 8000 to blunt end ligations. I have never
had this problem so I never tried the PEG addition. It is worth a try if
this is the only alternative. The other is to cut with PspA I or Xma I 
which leave an overhang. 
Good Luck,

Roger Anderson
Anderson Publications                   PO Box 36
rogera at                         Monrovia, CA 91017-0036
Anderson's Timesaving Comparative Guide: Restriction Enzyme Edition and
Anderson's Timesaving Comparative Guides on the Web
URL           Voice/FAX (818) 358-0579

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