DNA recovery from gels

Your Name Here Your_Name at ctpm.uq.edu.au
Tue Jul 11 23:51:14 EST 1995


bicfo at flinders.edu.au wrote:
>Gidday, fellow netters!
>I'm having truckloads of trouble trying to consistently recover DNA 
>(0.7-1.8kb) from low-melting point agarose gels. I've tried agarase digestion, 
>phenol extraction, Promega  WIZARD PCR columns  and 'squeeze-freeze' 
>techniques - all with limited success (none at all with the agarase!). Does 
>anybody have a foolproof method, or can anyone direct me to somewhere on the 
>net where different techniques might be discussed?
>Thanks, Colleen.
Qiagen has a rapid spin column tht is specifically designed for this purpose.  I 
haven't tried this product but commonly use other columns they produce and I've 
found them to be second to none.
Leon




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