Home-Made TA cloning
dkim at nmsu.edu
Tue Jul 11 10:55:04 EST 1995
In article <Stephen_Lasky-1007951408300001 at tonto-slip2.cis.brown.edu> Stephen_Lasky at brown.edu (Stephen R. Lasky, Ph.D.) writes:
>> Surely that should be ddTTP. If you use just dTTP then terminal transferase
>> will add umpteen 'T's.
>Duncan: I think that it IS dTTP. The terminal transferase that the
>previous writer is speaking of is the activity associated with Taq Poly,
>and, from my understanding, that ususally just adds a single non-template
>driven base. If I'm wrong, I'd appreciate knowing.
You are correct. Taq will add one dNTP onto the end of a double stranded
DNA, but will not add onto the end of a ssDNA, so the overhang is not a
substrate for further extension.
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