Transcription start site determination.

[Sean Stevens]

mb935645 at silver.shef.ac.uk (D L Clarke) wrote:
>Dear All,
>At present I am characterising a gene cloned from Aspergillus 
>nidulans.  For publication I need to determine the transcription
>start site. Does anybody know of any good methods or kits from
>which I can map the start site.
>
>Thanks
>
>Dave Clarke
>
>E-mail   D.L.Clarke at sheffield.ac.uk
>


Dave-

    Typically this is accomplished by standard primer extension assays.
I have not used a kit for this, but have had success with a fairly
abundant eukaryotic message using the protocol found in the 'red book'
also known as Current Protocols

                        -Sean

Sean Stevens  The Rockefeller University
stevens at rockvax.rockefeller.edu