Southern Hybridization Buffers?
stewart at ciit.org
Wed Jul 12 09:28:37 EST 1995
In article <3tpt6d$chq at agate.berkeley.edu>, Tjian lab
<insomnia at mendel.berkeley.edu> wrote:
> Here's my recipe for Church's buffer. IMHO, it's the best general
> purpose hyb buffer I've ever tried. Low background, easy to use, nice
> 0.5M Na phosphate, pH 7.2 (0.5M in Na, so 134g/l of Na2HPO4+7H2O,
> pH'ed with 4ml 85% phosphoric acid)
> 7% SDS
> 0.5mM EDTA
I also add 1%BSA to the above recipe.
These are my opinions only and do not reflect that of my company
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