Southern Hybridization Buffers?

Wendy Stewart stewart at ciit.org
Wed Jul 12 09:28:37 EST 1995


In article <3tpt6d$chq at agate.berkeley.edu>, Tjian lab
<insomnia at mendel.berkeley.edu> wrote:

> Here's my recipe for Church's buffer.  IMHO, it's the best general 
> purpose hyb buffer I've ever tried.  Low background, easy to use, nice 
> signals.
> 
> 0.5M Na phosphate, pH 7.2 (0.5M in Na, so 134g/l of Na2HPO4+7H2O,
>                             pH'ed with 4ml 85% phosphoric acid)
> 7% SDS
> 0.5mM EDTA
> 
I also add 1%BSA to the above recipe.

-- 
These are my opinions only and do not reflect that of my company



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