Lysing yeast spheroplasts

[Julie Wasylnka]

I am trying to lyse yeast spheroplasts in order to make yeast DNA.  I 
have a protocol which used DTT. I was wondering if I could substitute 2-ME 
for DTT and what concentration I should use? (The protocol suggests 1.8 
mM final DTT).

Thanks to all yeast experts,

Julie Wasylnka.