Lysing yeast spheroplasts

Julie Wasylnka wasylnka at
Wed Jul 12 15:50:51 EST 1995

I am trying to lyse yeast spheroplasts in order to make yeast DNA.  I 
have a protocol which used DTT. I was wondering if I could substitute 2-ME 
for DTT and what concentration I should use? (The protocol suggests 1.8 
mM final DTT).

Thanks to all yeast experts,

Julie Wasylnka.

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