Why probe hybridizes to vector?

[faerber at ubaclu.unibas.ch]

> 
> I routinely produce Southern probes by PCR using "insert-primers" to eliminate
> the presence of vector sequences.  However, I STILL see strong hybridization
> of this probe to vector (specifically Bluescript) sequence.  I believe this is 
> causing serious problems since I am trying to use this probe to screen a cDNA
> library constructed in Lambda Zap II (ie., Bluescript).
> 
> Any suggestions?
> 
> Amy Marion
> marion at smtplink.ipfw.indiana.edu

Do you have that problem with one special probe or with all your southern
probes? How long is our PCR-produced probe? 

Pit
faerber at ubaclu.unibas.ch