Sequencing gel solution premixes

[R. P. Grant +44 1 865 221018]

> I was wondering if a standard urea, acrylamide, TBE, water and TEMED solution 
> was stable during storage. I ask with regards to the companies  (e.g. National 
> Diagnostics) selling sequencing gel solutions which are ready to set after 
> the addtion of APS. 

Dunno about the TMED, but we used to make up a 10/9 stock of Urea/Ac in h2o, 
deionize and filter.  Kept for months at 4C (in the dark).  
Then we added 10XTBE (filtered, if necessary) and the AMPS + TEMED.  
I'd worry about the TBE ppting if stored cold.

rgds,

Richard
>  
-- 
Richard P. Grant MA DPhil      rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
FAX +44 1 865 69141            TEL +44 1 865 221018
http://sable.ox.ac.uk/~lady0266

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