Sequencing gel solution premixes
R. P. Grant +44 1 865 221018
rpgrant at molbiol.ox.ac.uk
Wed Jul 12 04:06:18 EST 1995
> I was wondering if a standard urea, acrylamide, TBE, water and TEMED solution
> was stable during storage. I ask with regards to the companies (e.g. National
> Diagnostics) selling sequencing gel solutions which are ready to set after
> the addtion of APS.
Dunno about the TMED, but we used to make up a 10/9 stock of Urea/Ac in h2o,
deionize and filter. Kept for months at 4C (in the dark).
Then we added 10XTBE (filtered, if necessary) and the AMPS + TEMED.
I'd worry about the TBE ppting if stored cold.
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
FAX +44 1 865 69141 TEL +44 1 865 221018
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