Yeasy PCR screening problem

Nigel Kenward mbxnjk at vax.nott.ac.uk
Wed Jul 12 02:45:00 EST 1995


Hi All,
Any ideas on how to do PCR colony screening on yeast ? I've tried freeze 
thawing and boiling but that doesn't seem to make the DNA accesible and 
we don't have French Presses, Hughes Pressure cells etc.
Any ideas,
Regards,
Nigel Kenward

               TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry      FAX:+44 (0)115 9422225
Queens Medical Centre      Email:mbxnjk at unicorn.nott.ac.uk
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