Yeasy PCR screening problem

Nigel Kenward mbxnjk at
Wed Jul 12 02:45:00 EST 1995

Hi All,
Any ideas on how to do PCR colony screening on yeast ? I've tried freeze 
thawing and boiling but that doesn't seem to make the DNA accesible and 
we don't have French Presses, Hughes Pressure cells etc.
Any ideas,
Nigel Kenward

               TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry      FAX:+44 (0)115 9422225
Queens Medical Centre      Email:mbxnjk at
Clifton Boulevard           
U.K.                   "Back off man, I'm a scientist!"  -- Bill Murray.

More information about the Methods mailing list